Announcement PDF ASX Announcement Abstract & Poster Presentation at Military Health System Research Symposium 2025 SYDNEY Australia, 5 August 2025: Recce Pharmaceuticals Ltd (ASX:RCE, FSE:R9Q) (the Company), the Company developing a new class of Synthetic Anti-infectives, will present a research Abstract and Poster at the 2025 Military Health System Research Symposium (MHSRS). The speaking session focuses on optimising medical readiness, MHS resilience, & biodefense across R&D for future pandemics. The abstract presents expanded data of RECCE® 327 demonstrating activity against multiple high-priority bioterrorism pathogens in laboratory testing. The pathogens tested included Bacillus anthracis (Anthrax), Francisella tularensis (Tularemia), Burkholderia mallei (Glanders), Burkholderia pseudomallei (Melioidosis), and Yersinia pestis (Plague), all classified as Category A and B bioterrorism threats by the United States Centers for Disease Control and Prevention (CDC). The Abstract & Poster publications will be accompanied by an in-person, oral presentation. Session Optimizing Medical Readiness, MHS Resilience, & Biodefense across R&D for Future Pandemics Title RECCE® 327: A Novel Countermeasure for High-Priority Bioterrorism Pathogens This poster presentation follows the Company’s Cooperative Research and Development Agreement (CRADA) with the United States Army Medical Research Institute of Infectious Diseases (USAMRIID), the United States’ Leading BSL-4 Biodefense Laboratory. USAMRIID will test Recce’s synthetic anti-infective RECCE® 327 (R327) against a panel of biodefence pathogens in USAMRIID’s established in vitro infection models. RECCE® 327: A Novel Countermeasure for High-Priority Bioterrorism Pathogens John Prendergast1, Michele Dilizia1, Alan Dunton MD1 ¹ Recce Pharmaceuticals, Brodie Hall Drive Technology Park, Bentley, WA, Australia Introduction Conclusions RECCE® 327 Activity RECCE® 327 (R327), a synthetic anti-infective developed by Recce Pharmaceuticals Ltd, has demonstrated significant activity against multiple high-priority bioterrorism pathogens in laboratory testing. This study was conducted by the Battelle Biomedical Research Center (BBRC) and involved Minimum Inhibitory Concentration (MIC) assays to evaluate R327’s efficacy. The pathogens tested included Bacillus anthracis (Anthrax), Francisella tularensis (Tularemia), Burkholderia mallei (Glanders), Burkholderia pseudomallei (Melioidosis), and Yersinia pestis (Plague), all classified as Category A and B bioterrorism threats by the U.S. Centers for Disease Control and Prevention (CDC). R327’s novel mechanism of action, targeting adenosine triphosphate (ATP) synthesis and bacterial membrane integrity, allows it to overcome traditional resistance mechanisms. The study underscores the importance of innovative anti-infectives like R327 in addressing bioterrorism and emerging infectious disease threats. Future research, including clinical and fieldbased validation, is crucial to establish R327 as a scalable and deployable solution for military and public health strategies. These findings contribute to ongoing efforts towards preparedness and resilience against biological threats. Against multiple high priority biopathogens Results The results demonstrated MIC values ranging from 75 to 600 μg/ mL, with R327 exhibiting potent activity against B. anthracis, F. tularensis, and Y. pestisDWFRQFHQWUDWLRQVRIƊvJP/7KHVH findings align with efficacy levels achieved in previous studies for ESKAPE pathogens. Higher concentrations of R327 were required to inhibit B. mallei (300-600 μg/mL) and B. pseudomallei (600 μg/ mL), showcasing the compound’s broad-spectrum activity against both Gram-positive and Gram-negative bacteria. The starting EDFWHULDOFRQFHQWUDWLRQVIRUHDFKSDWKRJHQUDQJHGIURP˜Ů WR˜Ů&)8P/ Pure (100%) R327 comprises an estimated 52,000 ug/ml of oligomers. There are approximately 1500 oligomers. Significant antimicrobial activity is most probably confined to a much smaller number of oligomers. Thus, the MIC values reported herein are comparatively high as they are calculated based on all oligomers present. Studies to identify the individual and active oligomer species and determination of their respective MIC values are ongoing. When the active oligomer species of R327 are identified and quantified, it is anticipated that the MIC values will significantly decrease. Learning Objectives At the end of the session, attendees will be able to: • Evaluate the efficacy of RECCE® 327 as a countermeasure against high-priority bioterrorism pathogens identified by the CDC. • Discuss the implications of synthetic anti-infectives in supporting the U.S. military’s biodefense readiness and resilience. • Identify opportunities to integrate novel anti-infective agents like RECCE® 327 into military and public health strategies to combat bioterrorism and antibiotic resistance. • MIC testing of R327 (in triplicate) • Study was conducted by Battelle Biomedical Research Center (BBRC). • MICs comparable to levels achieved for ESKAPE pathogen MICs tested Scan the QR code to access Recce’s website CDC Antimicrobial-Resistant Pathogens Tested • Strains resistant to levofloxacin (based on CLSI guidelines) were still susceptible to RECCE®327 • This suggests that pre-existing resistance genotypes are unlikely to confer resistance to RECCE®327 if used on clinical infections • Common resistance genotypes covered include: mexA (efflux), mcr1 (lipid syn), KPC (carbapenemase) • Strains were isolated from a variety of sources (provided by the CDC AR Bank), including but not limited to, wounds, blood, urine, and sputum. In most cases, multidrugresistance (MDR) was confirmed via sequencing by the CDC Antimicrobial Resistance Isolate Bank. MICs of selected panels of Gram-negative species was determined by Broth microdilution assays, performed with biological duplicates. The Company was also granted US$2M for burn wounds by the U.S. Department of Defense Congressionally Directed Medical Research Program (CDMRP) seeking to accelerate the development of R327G and evaluate it as a gel-based treatment to rapidly resolve burn wound infections. The MHSRS is the United States Department of Defence’s foremost scientific meeting. It provides a venue for presenting new scientific knowledge particular to military specific focuses in research and development. The MHSRS is the premier US military and select civilian meeting that draws approximately 4,000 attendees with focus to the unique medical needs of the Warfighter. The symposium will be held in Kissimmee, Florida 4-7 August 2025. A copy of the Poster and Abstract materials is available below and on the Company’s website. This announcement has been approved for release by Recce Pharmaceuticals Board.